Relationship between Th17-mediated immunity and airway inflammation in childhood neutrophilic asthma

Abstract Background The pathogenetic mechanisms of neutrophilic asthma are not well understood now. Whether T helper (Th)17-mediated immunity contributes to the pathogenesis in human is still under investigation. aim this study was explore relationship between Th17-mediated and airway inflammation childhood asthma. Methods Twenty-eight children with exacerbated without using any glucocorticoids were divided into three groups: eosinophilic (EA, n = 12) group, (NA, 10) group paucigranulocytic (PGA, 6) according induced sputum cytology. Ten healthy recruited as control (HC, group. Peripheral Th17 Th2 cells, expression Ki-67 peripheral cells detected by flow cytometry. mRNA retinoic acid-related orphan receptor γt (RORγt) blood mononuclear (PBMCs) qRT-PCR. concentrations IL-17, IL-8 IL-5 sputum, IL-17 plasma culture supernatant activated PBMCs measured ELISA. Results percentage Th all increased NA positively correlated neutrophil level each other. Also, RORγt positivity both concentration EA eosinophil Conclusions Both Th17- Th2-mediated involved There predominance proliferation